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Pharmaceutical calculations by alligation method

by egpat      Posted on 11-06-2017

Alligation is one of the simple and illustrative methods in pharmaceutical calculations for the pharmacy technicians. Dilution is a simple method for preparing a lower concentration from a solution of higher concentration. On the other hand, many of the times we require an intermediate concentration from two different concentrations where allegation plays its role.

The solutions should be mixed at a specific proportion in order to get required concentration.



Solutions can be mixed at a fixed proportion called as alligation ratio.

Here we will see few types of working examples on this method and different steps required for its calculation.

Let’s start our discussion with a simple example.

 Working example 1:

How many parts of 10% w/v solution and 4% w/v are to be mixed to produce 8%w/v solution.

Solution:

Step 1: Identify the data given.

Higher concentration=10% w/v

Lower concentration=4% w/v

Required concentration=8% w/v

What is required ?

    Alligation ratio=?

 

Step 2: Convert all the data into similar form and units

Here all the concentration terms are expressed in % w/v terms, hence no need to convert the format.

Step 3 : Apply alligation

Let’s indicate alligation ratio as H:L



Now to apply this method, subtract required concentration from higher concentration

H=8-4=4

Similarly, subtract lower concentration from required concentration

L=10-8=2

Working example 1 of alligation

Hence alligation ratio is=H : L =4 :2=2:1

Watch here a video explaining alligation method along with examples.



 Working example 2:

How many grams of 1.5 sp. gravity ointment should be mixed with 1.2 sp.gravity ointment to produce 15 gm of 1.3 sp.graviy ointment.

Solution:

Now let’s go another step in this pharmaceutical calculation i.e. to find out the weights of ointments to be mixed.

Step 1: Identify the data given.

Higher concentration (sp. gravity)=1.5

Lower concentration (sp. gravity)=1.2

Required concentration (sp. gravity)=1.3

What is required

   Alligation ratio=?

   Alligation volumes=?

Step 2: Convert all the data into similar form and units

Here again all the concentration terms are expressed in % w/v terms.


Step 3 : Apply alligation

Now applying the method,

            H=1.3-1.2=0.1

 

Similarly,

            L=1.5-1.3=0.2

Working example 2 of alligation

Hence alligation ratio is=H : L =0.1 : 0.2=1:2

Therefore 1 part of 1.5 sp. gravity ointment should be mixed with 2 parts of 1.2 sp.gravity ointment.

Step 4 : Calculate individual weight/volumes to be mixed

The weight of the higher concentration can be calculated by the following formula

Formula for calculation of higher concentration required for alligation

Since final weight of ointment is 15 g,the weight of the 1.5 sp gravity ointment required is 

Working example 2 of higher concentration required in alligation

 

Similarly, the weight of lower concentration can be calculated by the following formula.

Formula for calculation of lower concentration required for alligation

 

Hence the weight of 1.2 sp gravity ointment required is

Working example 2 of lower concentration required in alligation

 

Hence 5g of 1.5 sp gravity ointment should be mixed with 10g of 1.2 sp.gravity ointment to prepare 15g of 1.3 sp.gravtiy ointment.

 Working example 3:

Alligations – Soln volumes

How many millilitres of drug suspensions containing 80 mg/5 mL and  30 mg/5ml should be mixed with  to prepare 500 mL of a suspension of 10mg /ml

Solution:

Step 1: Identify the data given.

Higher concentration=80 mg/5ml

Lower concentration=30 mg/5ml

Required concentration=10 mg/ml

Final volume=500 ml

What is required

   Alligation ratio=?

   Alligation volumes=?


Step 2: Convert the two different concentrations into similar form and units.

Here two initial concentrations are given as gm per 5ml (80 mg/5 mL and 30 mg/5ml) whereas final concentration as gm/ml  (10mg /ml).

Let’s convert all in equal format.

Higher concentration is 80 mg/5 mL =16mg /ml

Lower concentration is 30 mg/5 mL =6mg /ml

 

Step 3 : Apply alligation

 

Now applying the method,

            H=10-6=4

Similarly,

            L=16-10=6

 

Working example 3 of alligation

Hence alligation ratio is=H : L =4 : 6=2:3

Therefore 2 parts of 80mg/5ml suspension should be mixed with 3 parts of 30mg/5ml suspension.

Step 4 : Get the volumes required

The fraction of higher concentration in mixture is=  2 / (3+2)

The fraction of lower concentration in mixture is=  3 / (3+2)

Final volume=500 ml

Hence the part/volume of higher concentration required is

Working example 3 of higher concentration required in alligation

Similarly the part/volume of lower concentration required is

Working example 3 of lower concentration required in alligation

 Working example 4:

Alligations – Soln volumes

How many millilitres of a 1:20 v/v solution of a drug is can be prepared from 100 mL of 6% v/v solution

Solution:

Step 1: Identify the data given.

Since the diluting solution is not given it can be considered as water. Hence lower concentration is treated as 0% v/v.

Higher concentration=6% v/v

Lower concentration=0% v/v

Required concentration=1:20 v/v

Higher concentration volume=100 ml

What is required

   Alligation ratio=?

   Alligation volumes=?

   Final volume=?

Step 2: Convert the two different concentrations into similar form and units.

Here we can clearly observe that all concentration terms are not expressed equal. Higher and lower concentrations are expressed in percentage (6% v/v) whereas required concentration is expressed in the ratio (1:20 v/v).

 

Let’s convert required concentration in to percentage expression.

The term 1:20 indicates 1 parts of solute in 20 parts of solution.

 

Hence 20 ml of solution contains 1ml of drug.

100 ml of solution contains

Conversion of ratio into percent expression

 

Now the concentrations are,

Higher concentration=6% v/v

Lower concentration=0% v/v

Required concentration=5% v/v

 

Step 3 : Apply alligation

 

Just as above,

Working example 4 of alligation

            H=5-0=5

Similarly,

            L=6-5=1

 

Hence alligation ratio is=H : L =5 : 1

 

Therefore 5 parts of 6% v/v solution should be mixed with 1 part of water to produce 5% v/v solution.

 

Step 4 : Calculate individual volumes to be mixed

 

The volume of the higher concentration  =100 ml

By substituting in the formula,

 

calculation 4 calculation of volume

 

Hence a total volume of 120 ml of 5 % v/v solution can be prepared from 100 ml of 6% v/v and 20 ml of 0% v/v (water).


So, that’s about the one of the interesting method in pharmaceutical calculations. Please share this article, if you like.




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